Counselling and analyses

Soil–Plant Analysis Laboratory, LASP

  • Electrochemical determinations (pH, conductivity)
  • Gas‑chromatography determinations (pesticide residues in soil and plant)
  • Ion‑chromatography determinations (nitrates in soil and plant)
  • Spectrometric determinations (metals in soil and plant by AAS)
  • Determination of plant‑available potassium in soil and total potassium in plant tissue
  • Spectrophotometric determinations (plant‑available phosphorus in soil, total phosphorus in plant; nitrates in soil and plant)
  • Determination of organic carbon and humus in soil (combustion and wet‑oxidation methods)
  • Determination of total nitrogen in soil and plant
  • Moisture determinations

Food Quality & Safety Testing Laboratory LICSA

The LICSA laboratory is authorised to perform the following physico‑chemical tests:

  • Water content
  • Sodium‑chloride content
  • Crude‑fat content
  • Fat acidity
  • Protein content
  • Nitrite / nitrate content
  • Organochlorine and organophosphorus pesticide residues (GC & GC‑MS)
  • Mycotoxins in food (TLC & HPLC)
  • Bitter compounds in hops (HPLC)
  • Conductometric value of hop cones, powders & pellets
  • Moisture of hops and hop products

Apicultural Products Quality‑Control Laboratory

A wide range of physico‑chemical analyses of honey and beeswax are available, including:

  • Water (refractometric)
  • Electrical conductivity
  • Ash content
  • Honey colour index
  • pH, free acidity, lactones, total acidity
  • HMF (White method)
  • HMF (HPLC)
  • Sensory (organoleptic) examination of honey
  • Diastase index & diastase activity
  • Reducing & easily hydrolysable sugars (Elser method)
  • Individual sugars (glucose, fructose, sucrose)
  • Pollen content (palynological analysis)
  • Water‑insoluble matter
  • Proline
  • Metals in honey

Agri‑Food & Biomedical Control and Expertise Laboratory  AGRIAL

  • Analyses of plants, foods, nutraceuticals (plant/mineral powders, tablets, capsules)
  • Quality‑test battery (QT) and special analyses (SA)
  • Fixed‑residue / dry‑matter and ash determinations
  • Direct pH or aqueous‑solution pH
  • Alkalinity and/or free‑acidity determinations
  • Spectroscopic “fingerprint” of samples in various solvents:
    • Aqueous extract
    • Polar extract (ethanol)
    • Non‑polar extract (ethyl acetate)
  • Spectroscopic identification of pigments (chlorophylls, carotenoids, polyphenols, flavonoids)
  • Fat‑soluble vitamins (A, E, D — separately or A+E, D₃)
  • Water‑soluble vitamins (B₁, B₂, B₆, B₁₂, niacin, folate, pantothenate)
  • Vitamin C (alone or combined with vitamins E & A)
  • Plant pigments: chlorophylls, carotenoids, flavonoids, anthocyanins
  • Total protein, total carbohydrates, total lipids
  • Lipid peroxides (MDA test)
  • Phytohormones
  • In‑vitro toxicity tests (viability, proliferation, respiration)
  • Mutagenicity screening
  • Inorganic phosphates; nitrates & nitrites
  • Organic nitrogen compounds (ATP, ADP, nitrogen bases)
  • Ammonia; vitamin C; H₂S (identification)
  • Peroxide index; chlorides

Water‑Testing & Noise‑Level Laboratory LIAZ

  • Electrochemical determinations (pH, conductivity)
  • Gas‑chromatography determinations (pesticide residues)
  • Ion‑chromatography determinations (cations & anions)
  • Atomic‑absorption spectrometry (metals)
  • Biochemical oxygen demand (BOD₅)
  • Chemical oxygen demand (COD_Cr)
  • Noise‑level measurements

Air‑Quality Analysis Laboratory

  • SO₂ in ambient air
  • NOx in ambient air
  • CO in ambient air
  • O₃ in ambient air
  • Suspended particulates (PM10 & PM2.5)
  • Volatile organic compounds (VOCs) in ambient air
  • Light‑intensity measurements

Animal Germplasm Certification & Cryostorage Laboratory

  • Macroscopic semen tests: volume, colour, odour
  • Microscopic semen tests: concentration, motility, viability, morphology
  • Microbiological tests: CFU count, bacterial strains, fungal agents
  • Biochemical assays: glucose, fructose, pyruvate, lactate
  • Morphological & morphometric evaluation of oocytes and embryos
  • Fluorescent staining & UV microscopy for embryo viability
  • Metabolic tests for embryo‑quality assessment
  • Specific‑marker determination (PCR‑RFLP)
  • Bovine‑semen freezing; ram/boar semen vitrification
  • Slow freezing of oocytes & embryos; long‑term straw storage in liquid‑nitrogen tanks

Feed Quality‑Control Laboratory

  • Proximate analysis of feeds (dry matter, crude protein, crude fat, crude fibre, crude ash, nitrogen‑free extract)
  • Mycotoxins – zearalenone
  • Pesticides – lindane

National Reference Laboratory for GMO Compliance

  • Integrated testing for Roundup‑Ready soya (event GTS 40‑3‑2) in raw materials and foods
  • DNA extraction (affinity‑column method)
  • Qualitative PCR testing
  • Quantitative real‑time PCR testing
  • GMO screening, identification & quantification (as above)
  • Paternity testing (animals or humans)

Techniques employed

  • Molecular identification (genetic fingerprinting) in plants
  • PCR‑based molecular analyses in plants and animals/humans
  • Molecular markers for disease diagnostics, genetic‑predisposition studies, marker‑assisted selection, biodiversity estimation, viral‑contamination detection, and gene‑expression quantification (all under validated protocols)

Regional Genotyping Laboratory for Farm Animals

  • DNA/RNA/protein extraction, quantification & purification from animal tissues
  • DNA fingerprinting (PCR‑RFLP, RAPD, AFLP, SSR)
  • Gene‑expression analysis (real‑time PCR, IEF, PAGE)
  • DNA sequencing; embryo sexing
  • Paternity certification of breeding stock
  • Early selection of elite breeders with genetic markers (caseins, β‑lactoglobulin, growth‑hormone, PIT‑1, prolactin, leptin)
  • Detection of mutant alleles causing genetic diseases
  • Biodiversity assessment with genetic markers
  • Authentication of milk, dairy and meat products (prevention of inter‑species adulteration)

Regional Laboratory for Apiculture Control & Diagnosis  DIA

  • Acarapis woodi (tracheal mites): microscopic ID
  • Varroa destructor & Tropilaelaps spp.: macro‑ & stereomicroscopic ID
  • Nosema apis spore count (direct microscopy)
  • Paenibacillus larvae detection (PCR)
  • Bee‑virus ID – CBPV & DWV (RT‑PCR)

Research, Surveillance, Diagnosis & Zoonosis‑Control Centre

Department of Parasitology & Parasitic Diseases

  • Epidemiological investigations in domestic & wild animals (clinical exam, coproscopy, ELISA, PCR, necropsy)
  • Testing antiparasitic molecules / plant extracts; chemoresistance; biological products
  • Coproparasitological, dermatological & serological diagnostics (methods listed)
  • Trichinellosis diagnosis (trichinelloscopy & artificial digestion)
  • Consultancy on control and prophylaxis of parasitoses & dermatological disorders

Department of Infectious Diseases

  • Bacterioscopy (Gram, Ziehl‑Neelsen, Giemsa, Tribondeau‑Fontana)
  • Bacteriology (culture, biochemical tests, API)
  • Antibiograms; serotyping (E. coli, Salmonella)
  • Wildlife & exotic‑animal diagnostics; autovaccine preparation
  • Molecular epidemiology (fingerprinting, PCR typing)
  • Vaccine/serum testing (OIE‑recognised)
  • Newcastle‑disease diagnosis & vaccine efficacy (HA & HI)
  • Haematology; humoral & cellular immunity assays; adjuvant testing
  • Porcine circovirus & equine infectious‑anaemia diagnostics; immune‑reactivity in reptiles etc.

Department of Microbiology

  • Special stains; morphometry; digital imaging
  • Aerobic/anaerobic cultures; selective/differential media; cultural‑feature archiving
  • Biochemical IDs (polytest media, API, mycobacteria panels)
  • Serological IDs; antibiotic/fungicide susceptibility tests; MIC/MBC; pathogenicity tests
  • Antibiotic‑level assays; bacteriocin production; innate‑immunity effectors
  • Antigen/antiserum production; electrophoresis & immunoelectrophoresis; statistics (GraphPad, Excel); consultancy

Department of Veterinary Hygiene of Products of Animal Origin & Public Health

  • Trichinelloscopy (compression & artificial digestion)
  • Hygienic & safety assessments of meat (total bacteria, spoilage or conditional pathogens)
  • Milk quality (fat, protein, lactose, TS, NFS, added water; pH, chloride, Cl–lactose index, conductivity, SCC, TBC, cytology, pesticide/antibiotic residues)
  • Honey quality & authenticity (water, adulteration, pollen spectrum)
  • Egg freshness tests
  • HACCP, GMP, SSOP: documentation, implementation & audit
  • Consultancy for setting up in‑house food labs; SAPARD project support
  • Species identification of animal‑origin products on request

 

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